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Plant genetic engineering and genetically modified crop breeding: history and current status

Xingchun WANG,Shujun CHANG,Jie LU,Rupert FRAY,Don GRIERSON,Yuanhuai HAN

《农业科学与工程前沿（英文）》 2017年第4卷第1期页码 5-27 doi: 10.15302/J-FASE-2017131

摘要： This review charts the major developments in the genetic manipulation of plant cells that have taken place since the first gene transfer experiments using Ti plasmids in 1983. Tremendous progress has been made in both our scientific understanding and technological capabilities since the first genetically modified (GM) crops were developed with single gene resistances to herbicides, insects, viruses, and the silencing of unde-sirable genes. Despite opposition in some parts of the world, the area planted with first generation GM crops has grown from 1.7 Mhm in 1996 to 179.7 Mhm hectares in 2015. The toolkit available for genetic modification has expanded greatly since 1996 and recently Nobel Laureates have called on Greenpeace to end their blanket opposition, and plant scientists have urged that consideration be given to the benefits of GM crops based on actual evidence. It is now possible to use GM to breed new crop cultivars resistant to a much wider range of pests and diseases, and to produce crops better able to adapt to climate change. The advent of new CRISPR-based technologies makes it possible to contemplate a much wider range of improvements based on transfer of new metabolic pathways and traits to improve nutritional quality, with a much greater degree of precision. Use of GM, sometimes in conjunction with other approaches, offers great opportunities for improving food quality, safety, and security in a changing world.

关键词： plant transformation transgene genetic modification

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核电站全生命周期数据管理框架体系构建与发展建议

任景莉,杨盼

《中国工程科学》 2022年第24卷第2期页码 152-159 doi: 10.15302/J-SSCAE-2022.02.012

摘要：

核工业正在经历数字化、智能化变革，核电数据呈指数型增长，数据逐渐成为核电站的重要资产，然而缺少贯穿核电站全生命周期的数据管理体系。本文综述了我国核电站全生命周期数据管理的研究现状，剖析了核电站全生命周期数据管理面临的需求与挑战，构建了围绕核电站全生命周期各个节点和业务流的数据管理框架。研究认为，建立核电站全生命周期数据管理体系当务之急是推进核电行业全面数字化，规范统一核电站全生命周期数据标准；依据数据类型对全生命周期数据进行分类管理，合理设计数据模型；科学制定数据质量管控政策、数据质量度量标准，确保核电站全生命周期数据安全。为此建议，加快建立数据一体化平台，对核电站中物项、业务数据、三维仿真数据等进行目录化组织，促进跨业务系统间数据的互联互通；将区块链技术应用到核电站全生命周期数据管理，实现核电站全生命周期数据的真实可溯；加强核电与数字孪生技术、基于模型的系统工程方法的融合应用，提高核电站智能化发展水平；建立健全核电站数据绿色管理政策体系，实现数据中心的低碳、高效、可持续发展。

关键词：数字化转型核电站全生命周期数据管理框架体系

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Removal, distribution and plant uptake of perfluorooctane sulfonate (PFOS) in a simulated constructed

Weichuan Qiao, Rong Li, Tianhao Tang, Achuo Anitta Zuh

《环境科学与工程前沿（英文）》 2021年第15卷第2期 doi: 10.1007/s11783-020-1312-3

摘要： Abstract • PFOS was removed by soil adsorption and plant uptake in the VFCW. • Uptake of PFOS by E. crassipes was more than that of C. alternifolius. • PFOS in wastewater can inhibit the removal of nutrients. • Dosing with PFOS changed the soil microbial community in the VFCW. A vertical-flow constructed wetland (VFCW) was used to treat simulated domestic sewage containing perfluorooctane sulfonate (PFOS). The removal rate of PFOS in the domestic sewage was 93%–98%, through soil adsorption and plant uptake, suggesting that VFCWs can remove PFOS efficiently from wastewater. The removal of PFOS in the VFCW was dependent on soil adsorption and plant uptake; moreover, the percentage of soil adsorption was 61%–89%, and was higher than that of the plants uptake (5%–31%). The absorption capacity of Eichhornia crassipes (E. crassipes) (1186.71 mg/kg) was higher than that of Cyperus alternifolius (C. alternifolius) (162.77 mg/kg) under 10 mg/L PFOS, and the transfer factor of PFOS in E. crassipes and C. alternifolius was 0.04 and 0.58, respectively, indicating that PFOS is not easily translocated to leaves from roots of wetland plants; moreover, uptake of PFOS by E. crassipes was more than that of C. alternifolius because the biomass of E. crassipes was more than that of C. alternifolius and the roots of E. crassipes can take up PFOS directly from wastewater while C. alternifolius needs to do so via its roots in the soil. The concentration of 10 mg/L PFOS had an obvious inhibitory effect on the removal rate of total nitrogen, total phosphorus, chemical oxygen demand, and ammonia nitrogen in the VFCW, which decreased by 15%, 10%, 10% and 12%, respectively. Dosing with PFOS in the wastewater reduced the bacterial richness but increased the diversity in soil because PFOS stimulated the growth of PFOS-tolerant strains.

关键词： Vertical-flow constructed wetland Perfluorooctane sulfonate Wetland plants Soil microbial community Effect Speciality: Wetlands Transformation Organic pollutants Phytoremediation Exposure assessment Sewage

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IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

《农业科学与工程前沿（英文）》 2021年第8卷第4期页码 635-644 doi: 10.15302/J-FASE -2020343

摘要：

The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of Eucalyptus tereticornis, Xanthium sibiricum, Artemisia argyi, Tupistra chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plant pathogenic fungi Aspergillus niger, Botrytis cinerea, Penicillium digitatum, P. expansum, P. italicumand Rhizopus stolonifer. All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v), 95% ethanol (v/v), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. T. chinensis may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.

关键词： Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis

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Transformation of triclosan by a novel cold-adapted laccase from

Yuanyuan Shi, Deyang Kong, Jiayang Liu, Junhe Lu, Xiaoming Yin, Quansuo Zhou

《环境科学与工程前沿（英文）》 2017年第11卷第3期 doi: 10.1007/s11783-017-0927-5

摘要： This work investigated the transformation of triclosan (TCS) by laccase produced by a pathogen isolated from rotten tomato. The pathogen was characterized as sp. FQ, belonging to subphylum . The laccase exhibited cold-adaptation with relatively high activity at 20°C. The laccase could effectively transform TCS. Approximately 62% TCS could be removed at dose of 1.0 unit·mL in 120 min. The reaction rate appeared to be to the concentration of the substrate, suggesting the laccase activity remained stable during the reaction. Transformation products of TCS were analyzed by mass spectrometry and it was revealed that TCS dimers were formed via radical coupling pathways. During this process, laccase catalyzed oxidation of TCS to form a radical intermediate is the rate limiting step. However, this step can be reversed by humic acid. Overall, the laccase showed great potential in the treatment of phenolic contaminants. Since laccase is widely presented in natural environment, this study also revealed an important pathway involved in the transformation of phenolic contaminants in the environment.

关键词： Laccase Botrytis cinerea Triclosan Transformation Kinetics

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FOOD SYSTEMS TRANSFORMATION: CONCEPTS, MECHANISMS AND PRACTICES

《农业科学与工程前沿（英文）》 2023年第10卷第1期页码 1-3 doi: 10.15302/J-FASE-2023491

摘要： FOOD SYSTEMS TRANSFORMATION: CONCEPTS, MECHANISMS AND PRACTICES

关键词： TRANSFORMATION SYSTEMS FOOD CONCEPTS MECHANISMS

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ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG

《农业科学与工程前沿（英文）》 2021年第8卷第4期页码 635-644 doi: 10.15302/J-FASE-2020343

摘要： The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of , , , and were evaluated against the mycelial growth of the plant pathogenic fungi , , and . All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol ( ), 95% ethanol ( ), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.

关键词： Botrytis cinerea food preservation plant diseases plant pathogenic fungi Tupistra chinensis

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TRANSFORMATION OF FOOD SYSTEMS: HOW CAN IT BE FINANCED?

《农业科学与工程前沿（英文）》 2023年第10卷第1期页码 109-123 doi: 10.15302/J-FASE-2023483

摘要：

● The analysis of financing for the transformation of food systems requires first a consideration of costs.

关键词： financial markets food systems development financing consumer expenditures public budgets Sustainable Development Goals

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The transformation of PAHs in the sewage sludge incineration treatment

Hui ZHANG,Le XU,Yifei ZHANG,Mengchan JIANG

《环境科学与工程前沿（英文）》 2016年第10卷第2期页码 336-340 doi: 10.1007/s11783-014-0766-6

摘要： The release of pollutants from sewage sludge in the treatment process is a worldwide tricky problem in the field of sludge treatment. In this paper, the release and reaction between monomers of polycyclic aromatic hydrocarbons (PAHs) in the sewage sludge incineration treatment were studied based on simulated experiments. The result indicates that the transformation may occurre between monomers of PAHs during their release in the treatment. Over 90% of total PAHs in sewage sludge are released at the the temperature of 300°C–750°C. The possible transformation of Naphthalene to indeno(1, 2, 3-cd)pyrene was related to the temperature of the treatment system. Here, we showed that the output rate of transformation reactions for indeno(1, 2, 3-cd)pyrene is 94% at 300°C. These findings, by identifying corresponding treatment conditions as well as techniques, can help to understand the reactions and control real outputs of PAHs in the treatment process.

关键词： release of monomers transformation of monomers PAHs sewage sludge incineration treatment

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An introduction to the medicinal plant genome project

Shilin Chen, Li Xiang, Xu Guo, Qiushi Li

《医学前沿（英文）》 2011年第5卷第2期页码 178-184 doi: 10.1007/s11684-011-0131-0

摘要： In recent years, genomics has developed rapidly with the application of next-generation sequencing technology. However, very few studies have been carried out on genomics for medicinal plants. This paper introduces the genome research of medicinal plants, including genome sequencing, assembly, annotation, and functional genomics, to set up the foundation for the development of natural medicines and the selection of cultivars with good agricultural traits. This study places the study on traditional Chinese medicine into the frontier field of life science.

关键词： genomics medicinal plant sequencing application

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面向数字化转型的“互联网 +”战略升级研究

何伟,张伟东,王超贤

《中国工程科学》 2020年第22卷第4期页码 10-17 doi: 10.15302/J-SSCAE-2020.04.002

摘要：

“互联网 +”行动计划推出5年来，新一代信息技术创新进一步加快，经济社会数字化转型呈现诸多新特征，这就要求深入研究构建转型新体系和探索转型新路径，谋划“互联网 +”行动举措演进升级。本文遵循技术产业变革的客观规律，基于技术经济理论范式，着重分析了当前技术、应用和政策等层面数字化转型新态势和转型前沿实践探索。研究表明，当前数字化转型技术体系和技术能力日益完善，新的转型目标蓝图逐步显现，信息技术和实体经济融合进入新阶段，面临着重构数字化转型框架体系，强化转型关键要素、突破转型关键环节的重要任务。对此建议，在“互联网 +”行动后续政策设计、国家“十四五”规划制定的过程中，发掘数字化要素潜力，推动数字化转型生态建设，构建转型创新网络，升级“互联网 +”政策保障体系，推动经济社会数字化转型迈向更高发展水平。

关键词：技术产业变革数字化转型互联网+ 产业升级

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HEMIPTERAN-TRANSMITTED PLANT VIRUSES: RESEARCH PROGRESS AND CONTROL STRATEGIES

《农业科学与工程前沿（英文）》 2022年第9卷第1期页码 98-109 doi: 10.15302/J-FASE-2021389

摘要：

About 80% of plant viruses are transmitted by specific insect vectors, especially hemipterans with piercing-sucking mouthparts. Many virus-transmitting insects are also important crop pests that cause considerable losses in crop production. This review summarizes the latest research findings on the interactions between plant viruses and insect vectors and analyzes the key factors affecting insect transmission of plant viruses from the perspectives of insect immunity, insect feeding, and insect symbiotic microorganisms. Additionally, by referring to the latest applications for blocking the transmission of animal viruses, potential control strategies to prevent the transmission of insect-vectored plant viruses using RNAi technology, gene editing technology, and CRISPR/Cas9+ gene-driven technology are discussed.

关键词： control strategies / feeding / immunity / insect vector / microorganism / plant virus

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Intelligent diagnosis methods for plant machinery

Huaqing WANG, Peng CHEN, Shuming WANG,

《机械工程前沿（英文）》 2010年第5卷第1期页码 118-124 doi: 10.1007/s11465-009-0084-z

摘要： This paper reports several intelligent diagnostic approaches based on artificial neural network and fuzzy algorithm for plant machinery, such as the diagnosis method using the wavelet transform, rough sets, and fuzzy neural network; the diagnosis method based on the sequential inference and fuzzy neural network; the diagnosis approach by the possibility theory and certainty factor model; and the diagnosis method on the basis of the adaptive filtering technique and fuzzy neural network. These intelligent diagnostic methods have been successfully applied to condition diagnosis in different types of practical plant machinery.

关键词： intelligent diagnosis neural network fuzzy algorithm adaptive filtering plant machinery

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Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

《农业科学与工程前沿（英文）》 2016年第3卷第2期页码 102-112 doi: 10.15302/J-FASE-2016100

摘要： Plants are frequently affected by pathogen infections. To effectively defend against such infections, two major modes of innate immunity have evolved in plants; pathogen-associated molecular pattern-triggered immunity and effector-triggered immunity. Although the molecular components as well as the corresponding pathways involved in these two processes have been identified, many aspects of the molecular mechanisms of the plant immune system remain elusive. Recently, the rapid development of omics techniques (e.g., genomics, proteomics and transcriptomics) has provided a great opportunity to explore plant–pathogen interactions from a systems perspective and studies on protein–protein interactions (PPIs) between plants and pathogens have been carried out and characterized at the network level. In this review, we introduce experimental and computational identification methods of PPIs, popular PPI network analysis approaches, and existing bioinformatics resources/tools related to PPIs. Then, we focus on reviewing the progress in genome-wide PPI networks related to plant–pathogen interactions, including pathogen-centric PPI networks, plant-centric PPI networks and interspecies PPI networks between plants and pathogens. We anticipate genome-wide PPI network analysis will provide a clearer understanding of plant–pathogen interactions and will offer some new opportunities for crop protection and improvement.

关键词： plant–pathogen interactions systems biology omics plant immunity protein–protein interaction network

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Causations of failure in megaprojects: A case study of the Ajaokuta Steel Plant project

Oluwole Alfred OLATUNJI

《工程管理前沿（英文）》 2018年第5卷第3期页码 334-346 doi: 10.15302/J-FEM-2018011

摘要：

This paper uses project organizational theories to draw lessons from a historic megaproject, the Ajaokuta Steel Plant (ASP). Archival reports on the ASP were explored to identify the unique attributes of the project; the political wrangling that underplayed its evolution, its economic significance and organizational impacts. Findings suggest the goals of the ASP project were, and still are, unambiguous. Failure occurred as socio-political forces aggravated the project’s complex milestones. Stakeholders were impatient with pre-project investigations. During planning, owners ignored opinions that were contrary to their expectations. While delays lingered, pressures from the global economy weakened the project’s motivation to succeed. These combined to turn the project’s outcomes into a chaotic situation that triggered dire implications. Despite about 1400% overrun in cost, the success achieved on the plant was 28% at commissioning. Contractors remained on site until eight years after commissioning. Six key elements of the 482 items in the ASP project contract were not delivered nearly 40 years on. A simplistic look at these suggests poor planning is the main problem. However, planning issues is not entirely strange in greenfield projects. The paper draws strength from project organization theories to explain what was poor about the planning. Socrates’ generic management theory was used to explain the role of leadership in the failure of the ASP project. McGregor’s Theory X and Theory Y explain the significance of stakeholders’ integration in megaprojects. Systems and chaos theories were used to explain the sensitivity of the ASP project to uncertainties. Narratives on these combine well to inspire stakeholders of megaprojects on where and how to seek courage in making effective plans that can help achieve success in complex projects. While normative literature only recognizes project success in a definitive perspective, this study provides insights from failure as an instrument to trigger sublime reflections.

关键词： industrial projects megaprojects Nigeria project organizational theories steel plant